The pumps exhibited good repeatability in flowrates and functioned inside a cell incubator (at 37 ☌ and 95 % humidity) for seven days without noticeable issues in the performance. To demonstrate the forward compatibility, we developed a flowrate calibration tool that was readily interfaced with the developed pump system. This kit was designed with modular components (i.e., each component followed a standardized unit) to achieve (1) customizability (users can easily reconfigure various components to comply with their experiments), (2) forward compatibility (new parts with the standardized unit can be designed and easily interfaced to the current kit), and (3) easy replacement of the parts experiencing wear and tear. The assembled pumps offered a flowrate of 0.02 – 727.3 μL/min, and the smallest pump assembled with this kit was 20 × 50 × 28 mm. We demonstrated fabricating two variants of pumps with different sizes and operating flowrates using the developed kit. Herein, we developed a cost-effective kit to build a micro-peristaltic pump (approximately 50 USD) consisting of 3D-printed and off-the-shelf components. These results can lay the foundation for the functional, fast, and low-cost programmed mini-pump in PCR or other applications for trace measurements.Ĭommercially available peristaltic pumps for microfluidics are usually bulky, expensive, and not customizable. The results proved that the custom-made three-dimensional programmed mini-pump has a stronger reproducibility compared with manual pipette (ORF1ab: 24.06 ± 0.33 vs. Besides, the contrast test between three-dimensional programmed mini-pump and manual pipette was conducted with the ORF1ab and pGEM-3Zf (+) genes in qPCR. With the weighting calibration before and after pipetting operation, the error of the pipette in 10 μL (0.2%), 2 μL (1.8%), and 1 μL (5.6%) can be obtained. In view of the cost of the current automatic pipetting pump being higher, which is difficult to use in a regular lab, this paper designed and assembled a three-dimensional programmed mini-pump with the common parts and components, such as PLC controller, motor, microinjector, etc. Programmed mini-pumps play a significant role in various fields, such as chemistry, biology, and medicine, to transport a measured volume of liquid, especially in the current detection of (COVID-19) with PCR. µPump provides researchers, students, and startups with a cost-effective solution for precise fluid control. µPump can be used for two-phase droplet microfluidics, single-phase microfluidics, gaseous flow microfluidics and any other applications requiring precise fluid handling. The design rationale is presented, together with documented design details and software, so that the system may be replicated or customized to particular applications. $5000 USD) is approximately a quarter, or a third of the cost of buying a high-end commercial system, respectively. $3000 USD) or an eight-channel µPump (approx. Material for building a four-channel µPump (approx. The settling time to reach 2 bar from zero and stabilize is less than 2 s. The pressure accuracy, stability, and resolution are 0.09%, 0.02%, and 0.02% of the full span, respectively. This mechatronic system is coined ‘µPump,’ and its performance rivals that of commercially available systems, at a fraction of the cost. Check out the related items below for a compatible wall-wart power supply.An open-source precision pressure pump system and control software is presented, primarily designed for the experimental microfluidics community, although others may find additional uses for this precision pressure source. The on-board power regulator can handle anything from 7 to 15VDC. You can power the RedBoard over USB or through the barrel jack. This version adds an SMD ISP header for use with shields. We've also broken out the SDA, SCL and IOREF pins that showed up on the UNO R3, so the RedBoard will be compatible with future shields. RedBoard has all of the hardware peripherals you know and love: 14 Digital I/O pins with 6 PWM pins, 6 Analog Inputs, UART, SPI and external interrupts. The RedBoard can be programmed over a USB Mini-B cable using the Arduino IDE: Just plug in the board, select "Arduino UNO" from the board menu and you're ready to upload code. The SparkFun RedBoard combines the simplicity of the UNO's Optiboot bootloader (which is used in the Pro series), the stability of the FTDI (which we all missed after the Duemilanove was discontinued) and the R3 shield compatibility of the latest Arduino UNO R3. Each board is a bit different and no one board has everything we want, so we decided to make our own version that combines all our favorite features. At SparkFun we use many Arduinos and we're always looking for the simplest, most stable one.
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